Objectives: To determine the effects of education on knowledge, attitude and practice status regarding cervical cancer, its prevention and screening among registered nurses working at tertiary care hospitals. Methods: The quasi-experimental study was conducted in 2016(15th June-15th July) at Civil Hospital, Karachi, and Dow University of Health Sciences, Karachi, and comprised registered nurses enrolled through non-probability convenience sampling. Baseline demographic characteristics and knowledge, attitude and practice levels of the nurses was gathered using a self-structured pretested and validated questionnaire. An education session on, "prevention and screening of cervical cancer" was conducted and its effectiveness was determined using the same questionnaire post-intervention. The difference in pre- and post-intervention values were determined. P0.05). The difference in knowledge scores were significant in all age groups, marital status, work experience and education (p<0.05 each). Conclusion: There was a highly significant impact of education on the knowledge level of the subjects.