Objective: The study was conducted for knowing the spectrum of organisms and their sensitivity patterns against antibiotics. Study Design: It was cross sectional study. Place and Duration of Study: 100 cases of clinical sepsis fulfilling the inclusion criteria were enrolled in study. Duration of study was 6 months (from 1st January 2019 to 30 June 2019). Study was conducted at Bhatti International Teaching Hospital Kasur. Material and Methods: Clinical features were noted on proforma. CBC, CRP, Blood C/S were sent on admissions and results were entered and analyzed using the SPSS 23 software. Results: Gram Negative organisms were predominant among 32 positive cultures out of total 100. E. coli, klebsiella, pseudomonas, Staphylococcus aureus and epidermidis were among isolates. Ampicillin, gentamicin were resistant to most organisms. Ceftazidime, cefotaxime and amikacin were having intermediate sensitivity. Vancomycin and meropenem were most sensitive to isolated organisms. Conclusion: Gram negative sepsis is predominant and E. Coli is leading cause of sepsis. Amikacin and cephalosporin should be used as first line, if no response then vancomycin and meropenem combination should be used. A periodic survey regarding Culture sensitivities ensures best treatment options against neonatal sepsis.