Objective: To determine the frequency of Pre-analytical errors in sample collected for coagulation profile. Material and Methods: This descriptive cross-sectional study was conducted at Department of Pathology, Combined Military Hospital Multan Cantt from 14th Oct 2019 to 31st March 2020. We have collected the samples for coagulation profile from Main ITC, CCU, Surgical ITC, Pediatrics, Medical and Gynecology wards and divided these samples into four categories (Cat); Cat 1: Properly filled, Cat 2: Overfilled, Cat 3: Under Filled and Cat 4: Clotted. Data was entered in Microsoft excel for compilation. Results: Age range was from 1 to 83 years, mean age of 57.4 ± 7.3 years. Among them, 3881 (49.1%) were male and 4026 (50.9%) were females. Out of (7907) samples 6656 samples were properly filled, (927) were over filled, (230) samples was under filled and 94 samples was clotted. In Cat 2, 276 cases (30%), in Cat 3, 36.5% samples showed abnormal results. Conclusion: Frequency of Pre-analytical variables in sample collected for coagulation profile is quite high.