Hepatitis C virus (HCV) was identified as major causative agent of non-A, non-B hepatitis. Comparison of numerous complete and partial nucleotide sequences of HCV isolates has revealed a marked genetic heterogeneity suggesting the existence of HCV genotypes. On the basis of sequence variation in both the coding and non-coding regions, numerous classification systems have been proposed. Now HCV has been classified into six genotypes and numerous subtypes. Recent studies have pointed out that different HCV genotypes are associated with different profiles of pathogenicity, infectivity, and response to antiviral therapy. We used typing system based on PCR by using genotype specific primers focused on HCV core region. Genotype specific primers were designed for genotype 1a, 1b, 2a, 2b, 3a, 3b and 4.Ninety suspected HCV samples were collected, out of which 66 were found to be positive for HCV.The frequency of occurrence of genotype 2a, 2b 3a and 3b was found 13.6%, 4.56%, 66.6% and 15% respectively. This study in correlation with other studies on HCV genotyping shows that the most prevalent genotype in Punjab province is 3a.This study will help in correlating efficacy of interferon therapy with different HCV genotypes.