MORPHOLOGICAL CHARACTERIZATION, MULTIVARIATE ANALYSIS AND MICROPROPAGATION OF HYBRID ROSE (Rosa indica L.) GERMPLASM

Monis Hussain Shah; Riaz Ur Rahman; Abid Mahmood; Muhammad Usman; Sajida Bibi

doi:10.21162/PAKJAS/21.9122

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MORPHOLOGICAL CHARACTERIZATION, MULTIVARIATE ANALYSIS AND MICROPROPAGATION OF HYBRID ROSE (Rosa indica L.) GERMPLASM

Author(s)

Monis Hussain Shah Directorate of Floriculture (T&R),Punjab, Lahore, Pakistan, 54000,

Riaz Ur Rahman Directorate of Floriculture (T&R),Punjab, Lahore, Pakistan, 54000,

Abid Mahmood Directorate of Floriculture (T&R),Punjab, Lahore, Pakistan, 54000,

Muhammad Usman Institute of Horticultural Sciences, University of Agriculture,Faisalabad Pakistan, 38000,

Sajida Bibi Nuclear Institute for Agriculture and Biology, Faisalabad Pakistan,38000,

Abstract

Rose is a highly economically important ornamental plant being globally used for aesthetic purposes since ancient times. In Pakistan, during last seventy years hundreds of Hybrid varieties were introduced and adopted for cut-flower production and landscape beauty. Several spontaneous mutants may also have originated during clonal propagation. In 2008, a comprehensive survey of Punjab areas was conducted and several hybrids were collected and planted in semi sub-tropical area (Rawalpindi/Islamabad). The varieties with stable phenotypic characters were further subjected to morphological analysis following UPOV-Rose descriptors during 2017 to 2019. Morphological diversity was analyzed using principal component analysis (PCA), cluster analysis and a large ratio of the characters was considered as dominant. The narrow range of variation was found within germplasm of Hybrid accessions and traits (11.43% and 10.14%) on factor plains (Factor 1 × Factor 2). The leading accessions 33, 34 and 6 were selected for their desirable plant attributes such as better flower size (cm), stem length (cm) and early flower production. Establishment of in vitro clonal propagation was required for efficient multiplication. The explants were surface sterilized by pre-soaking in Topsin-M fungicide and sequential use of HgCl2 and NaOCl. Phenolic exudation was controlled using 1% Citric acid solution. Among selected accessions, #33 performed better for efficient establishment of cultures and in vitro clonal multiplication. Hence, it may be used for further clonal propagation using tissue culture for commercial production. This is first report of the adoptability studies of different HT-Rose accessions in Pakistan and their capacity to be used in selection and breeding programs.

Publication Details

Page(s) 51-59

DOI 10.21162/PAKJAS/21.9122

Published Journal: Pakistan Journal of Agricultural Sciences, Volume: 58, Issue: 1, Year: 2021

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Keywords

principal component analysis, Phenotype, in vitro, Characterization, Adoptability

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