This study quantified the effect of major factors affecting the milk production in buffaloes. The important factors considered for the estimation of milk production included the value of dry fodder, the value of green fodder, the value of concentrate, number of lactations and the value of labour hours. A production function for milk was estimated by employing Ordinary Least Square (OLS) regression. In the estimated model among all the relevant variables, green fodder, lactation number and labour hours were found to be the influential factors affectingmilk production in this species.