The aim of this study was to determine the use of eye protection by dental staff and patients as well as to highlight the significance o f eye protection in dental practice setting, with regard to adequate protection and its use at appropriate times. This study was conducted at Royal Medical Services affiliated hospitals in Jordan from January 2008 to December 2008. Questionnaires were sent to 100 dentists (with different specialties), chosen at random, to update the information on the eye protection, the preferred methods of eye protection and any experience of ocular injury. The patients data and information were collected from the patients who filled the proforma during their visits to dental clinics. Four hundred patients were included in the study, 60% were males and 40% females, with age rage 5-85 years. 30% of the adults and 20% children used eye protection. Eighty per cent of dentists wore eye protection routinely, but their choice of protection was not always adequate and not worn for all procedures. Sixty percent of dentists had experience of ocular trauma or infection which occurred during variety ofprocedures; 73% of these injuries resulted from not wearing eye protection. In contrast less than one third of the dental nurses (DNs) used protection routinely, particularly when cleaning contaminated instruments. However, hygienists did wear eye protection for the majority of their patient work (95%). It was concluded that eye protection use by all dental staff and patients was below the recommended guidelines. Risks encountered within the dental environment do cause harm to the unprotected eye which can be greatly reduced or even eliminated by improving the uptake of suitable eye protection. Several choices for eye protection are available. Each individual dentist is responsible for the uptake of eye protection for all persons in their dental procedure environment.