Cellulase Production by Trichoderma viride Fermentation using Response Surface Methodolog y in Submerged

Muhammad Irfan; Muhammad Nadeem; Misbah Ghazanfar; Qurratulain Ahmad; Shaukat Ali; Farzana Siddique; Zarina Yasmeen; Marcelo Franco; Tehreem Zahra

doi:10.17582/journal.pujz/2020.35.2.223.228

| The potential of Trichoderma viride for cellulase production using seed pods of silk cotton tree as substrate in submerged fermentation by response surface methodology has been investigated. Three variables like substrate concentration, peptone and KH 2PO4 were optimized at three levels.The optimum carboxymethylcellulase (CMCase) activity and filter paper cellulase (FPase) activity was obtained after 96 hours of incubation with optimum conditions of media containing 5% substrate concentration, 0.05% peptone and KH2PO4 concentration of 0.5% with pH 5.0 at incubation temperature of 30?C. The model proposed was found significant. hTe cellulase produced could be potentially used in industries especially for biofuel production. Novelty Statement | This is first report on cellulase production using Bombyx cieba as substrate in submerged fermentation by Trichoderma viride.