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Differences in Gene Expression Profiles between Equine and Bovine Thyroid Epithelial Cells Attacked by FMDV

Author(s)
Yanqin Wang Jining Normal University,Wulanqab, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang, China
Jianyong Zhu Ulanqab Vocational College, Wulanqab, China
Yong Zhang Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang China
Jie He Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Xianyang China
Abstract
Horses (equine) are common livestock, which are unusually insensitive to the Foot-and-mouth disease virus (FMDV). Similarities and differences between bovine and equine attacked by FMDV may uncover the reasons for this insensitivity. In the current study, ETYECs (equine thyroid epithelial cells) underwent apoptosis earlier and at a higher rate than did BTYECs (bovine thyroid epithelial cells) after FMDV infection. Further analysis and verification of gene expression profiles showed significant differences in the PI3K-Akt signaling pathway and Toll-like receptor pathway between BTYECs and ETYECs. Importantly, FMDV activated the PI3K-Akt-Bim and PI3K-Akt-Bad/Bcl2 axes in ETYECs and BTYECs, respectively. The BTYECs became insensitive when expression patterns of TLR3 and MyD88 were changed and following changes in IRF7. Related changes in Bad, Bcl2 and Bim may indicate a complementary relationship between the PI3K-Akt signaling pathway and Toll-like receptor pathway. In summary, FMDV triggers apoptosis and IFNa/ß anti-viral effects in ETYECs, but has antiapoptotic effects and inhibits interferons in BTYECs making them suitable for production of FMDV. These results provide new insights into antiviral mechanisms. 
Publication Details
Page(s) 1033-1040
DOI 10.17957/IJAB/15.1530
Published Journal: International Journal of Agriculture and Biology, Volume: 24, Issue: 4, Year: 2020

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Keywords
equine, signal pathway, Foot-and-mouth disease virus
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