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Microbiological Profile and Antimicrobial Susceptibility Pattern of Microorganisms Isolated from Endotracheal Tube Tips and Tracheal Aspirates Specimens: A Hospital Based Study

Author(s)
Alia Batool Department of Pathology, Fatima Memorial Hospital, FMH College of Medicine and Dentistry Shadman, Lahore, Pakistan
Sana Ashiq Center for Applied Molecular Biology, University of the Punjab,Lahore-, Pakistan
Durre Shahwar Lone .Department of Pathology, Fatima Memorial Hospital, FMH College of Medicine and Dentistry Shadman, Lahore, Pakistan
Ayaz Lone Department of Pathology, Fatima Memorial Hospital, FMH College of Medicine and Dentistry Shadman, Lahore, Pakistan
Kanwal Ashiq Faculty of Pharmaceutical Sciences, Superior College,Raiwind Road, Lahore-, Pakistan
Sabiha Riaz Department of Pathology, Fatima Memorial Hospital, FMH College of Medicine and Dentistry Shadman, Lahore, Pakistan
Abstract
Objective: To determine the microbiological profile and antimicrobial susceptibility pattern of microorganisms isolated from specimens of tracheal aspirate and endotracheal tube (ETT) tip. Methods: A descriptive cross-sectional study was conducted in a tertiary care hospital, Lahore, Pakistan during July 2016 to July 2017. The laboratory records of ETT tips and tracheal aspirate specimens was retrospectively reviewed. Standard microbiological procedures were followed for the isolation and the identification of microorganisms. Clinical and Laboratory Standards Institute (CLSI) 2016, guidelines were used for the antimicrobial susceptibility testing. Results: A total of 126 organisms were isolated from 121 specimens [Tracheal Aspirate 103 (85.1%) and ETT Tip 18 (14.9%)]. Most of the organisms were gram-negative 98 (77.78%), 17 (13.49%) were gram-positive and 11 (8.73%) were yeast. Of 98 gramnegative organisms, Acinetobacter spp was found in majority 61 (62.24%) followed by Klebsiella spp in 17 (17.34%), and Pseudomonas spp in 12 (12.24%). Of 17 gram-positive samples, Streptococcus spp was found in majority 6 (35.29%), followed by Methicillin-Resistant Staphylococcus aureus (MRSA) in 5 (29.41%), Staphylococcus epidermidis in 2 (11.76%), and Streptococcus Group D in 1 (5.88%). Candida was the only specie found in yeast. Acinetobacter was found highly resistant to all antibiotics except doxycycline, colistin and polymixin-b. Pseudomonas was sensitive to all the antibiotics except to ceftazidime (100% resistant). Klebsiella and MRSA showed high resistance to all the tested antibiotics. Conclusion: Gram-negative was the most common isolated bacteria from ETT tips and tracheal aspiration. Moreover, the specific isolates pattern of antimicrobial susceptibility showed a high resistance to widely used antibiotics.
Publication Details
Page(s) 32-37
DOI DOI not available
Published Journal: Journal of Dow University of Health Sciences, Volume: 14, Issue: 1, Year: 2020

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Keywords
Antimicrobial Susceptibility, Endotracheal Tube (ETT) tip, Bacterial infection, Tracheal Aspirate
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