iTRAQ-Based Quantitative Differentially Expressed Proteins Wolfberry (Lycium barbarum) from Different Habitats in China of

Tingting Kou; Yinhong Niu; Yanli Fan; Jinghua Chen; Lina Han

doi:10.17957/IJAB/15.1357

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iTRAQ-Based Quantitative Differentially Expressed Proteins Wolfberry (Lycium barbarum) from Different Habitats in China of

Author(s)

Tingting Kou Ningxia Key Laboratory for Food Microbial-Applications Technology and Safety Control,Yinchuan,P.R. China; School of Agriculture, Ningxia University,Yinchuan,P.R. China

Yinhong Niu Ningxia Key Laboratory for Food Microbial-Applications Technology and Safety Control,Yinchuan,P.R. China; School of Agriculture, Ningxia University,Yinchuan,P.R. China

Yanli Fan Ningxia Key Laboratory for Food Microbial-Applications Technology and Safety Control,Yinchuan,P.R. China; School of Agriculture, Ningxia University,Yinchuan,P.R. China

Jinghua Chen Ningxia Key Laboratory for Food Microbial-Applications Technology and Safety Control,Yinchuan,P.R. China; School of Agriculture, Ningxia University,Yinchuan,P.R. China

Lina Han Ningxia Key Laboratory for Food Microbial-Applications Technology and Safety Control,Yinchuan,P.R. China; School of Agriculture, Ningxia University,Yinchuan,P.R. China

Abstract

This study was to research the proteome differences of 'Ningqi No. 1' wolfberry (Lycium barbarum L., also known as 'goji') from Zhongning Ningxia and other four habitats in China (Jinghe, Xinjiang, Urat Front Banner, Inner Mongolia, Guazhou, Gansu, and Delingha, Qinghai) by iTRAQ and 2D LC-MS/MS. A total of 4852 proteins were identified with at least one unique peptide. And 166 (Inner Mongolia/Ningxia), 446 (Xinjiang/Ningxia), 1015 (Gansu/Ningxia) and 996 (Qinghai/Ningxia) DEPs showed a significant (P < 0.05) change (=1.2 or =0.83) in relative abundance. Gansu/Ningxia and Qinghai/Ningxia have more DEPs than Xinjiang/Ningxia and Inner Mongolia/Ningxia. Bioinformatic and functional analysis showed that DEPs were mainly bound up with GO (gene ontology) functional items, such as 'metabolic process', 'cellular process' and 'single-organism process'; DEPs were mainly involved in the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway of 'carbon metabolism', followed by 'biosynthesis of amino acids' and 'protein processing in endoplasmic reticulum'. These results suggested that wolfberry in Ningxia had little difference with Inner Mongolia and Xinjiang, but had great difference with Gansu and Qinghai, and their differences were mainly in 'metabolic progress'. This research may supply a new visual angle to understand the differences of wolfberry from different habitats.

Publication Details

Page(s) 820-828

DOI 10.17957/IJAB/15.1357

Published Journal: International Journal of Agriculture and Biology, Volume: 23, Issue: 4, Year: 2020

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Keywords

KEGG (Kyoto Encyclopedia of Genes and Genomes), GO (Gene Ontology), Differentially expressed proteins (DEPs), Wolfberry (Lycium barbarum L

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