Micropropagation through apical shoot explants and morphogenic potential of different explants of Saussurea lappa: An endangered medicinal plant.

Samin Jan; MuhammadAnwar Sajad; SafdarHussain Shah; Ghulam Farooq; Hazrat Ali; Zaib-Un-Nisa

doi:10.19045/bspab.2018.700220

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Micropropagation through apical shoot explants and morphogenic potential of different explants of Saussurea lappa: An endangered medicinal plant.

Author(s)

Samin Jan Department of Botany, Islamia College Peshawar (ICP), Peshawar-Pakistan

MuhammadAnwar Sajad Department of Botany, Islamia College Peshawar (ICP), Peshawar-Pakistan

SafdarHussain Shah Institute of Biotechnology and Genetic Engineering (IBGE), Khyber Pakhtunkhwa Agriculture University, Peshawar-Pakistan

Ghulam Farooq Department of Genetics, Hazara University, Mansehra-Pakistan

Hazrat Ali Department of Zoology, University of Malakand, Chakdara 18800, Dir Lower-Pakistan

Zaib-Un-Nisa Department of Botany, Islamia College Peshawar (ICP), Peshawar-Pakistan

Abstract

Saussurea lappa is a medicinal plant, which is used against a lot of diseases. Due to over exploitation for commercial and medicinal purposes, this plant is now endangered. Current study was carried out to develop a protocol for micropropagation and to determine morphogenic potential of different explants for callus induction of S. lappa, with the objective to develop plants in short time and large number. The explants were properly sterilized with 0.1% mercuric chloride for 1, 2 and 3 minutes. Maximum sterilization was observed with 3 minutes but decreased explants survival. However, 85% explants survived with 1-minute treatment. Benzyl amino purine and indolebutyric acid at 1.0 mg L-1 concentration produced 90% shoots and 93.66% roots respectively. Among the different explants, seedling and root explants were found more efficient in callus induction compared to other explants. Callus initiation occurred after 3 days of culturing of shoot and seedling followed by petiole (6 days) whereas lamina explants took 15-20 days. After 7 subsequent subcultures, the highest relative growth rate of 0.141 week-1 of callus was noted in seedling explants followed by root 0.098 week-1 and petiole 0.082 week-1 whereas lamina showed significantly low relative growth rate of 0.016 week-1. Tissue culture technique should be used for conservation of endangered medicinal plants such as Saussurea lappa.

Publication Details

Page(s) 585-592

DOI 10.19045/bspab.2018.700220

Published Journal: Pure and Applied Biology, Volume: 8, Issue: 1, Year: 2019

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Keywords

Callus induction, Saussurea lappa, Explants, sterilization, Relative growth rate, Micropropagation