Expression Pattern Analysis of Core Regulatory Module SHPS-FUL Transcripts in Rapeseed Pod Shattering.

Muhammad Yasin; Romana Shahzadi; Muhammad Riaz; Mahideen Afridi; Wajya Ajmal; Obaid Ur Rehman; Nazia Rehman; Ghulam Muhammad Ali; Muhammad Ramzan Khan

Muhammad Yasin PARC Institute of Advanced Studies in Agriculture, National Agricultural Research Center, Park Road, Islamabad, Pakistan

Romana Shahzadi PARC Institute of Advanced Studies in Agriculture, National Agricultural Research Center, Park Road, Islamabad, Pakistan

Muhammad Riaz National Centre for Bioinformatics, Quaid-i-Azam University, Islamabad, Pakistan

Mahideen Afridi National Centre for Bioinformatics, Quaid-i-Azam University, Islamabad, Pakistan

Wajya Ajmal National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Center, Park Road, Islamabad, Pakistan

Obaid Ur Rehman National Centre for Bioinformatics, Quaid-i-Azam University, Islamabad, Pakistan

Nazia Rehman National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Center, Park Road, Islamabad, Pakistan

Ghulam Muhammad Ali National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Center, Park Road, Islamabad, Pakistan; PARC Institute of Advanced Studies in Agriculture, National Agricultural Research Center, Park Road, Islamabad, Pakistan

Muhammad Ramzan Khan National Centre for Bioinformatics, Quaid-i-Azam University,Islamabad, Pakistan; National Institute for Genomics and Advanced Biotechnology, National Agricultural Research Center, Park Road, Islamabad, Pakistan; PARC Institute of Advanced Studies in Agriculture, National Agricultural Research Center, Park Road, Islamabad, Pakistan

Non-synchronous pod shattering is the main cause of yield losses in canola. The expression of SHATTERPROOF1/2 (SHP1and SHP2) and FRUITFULL (FUL) MADS-box genes is fundamental to fruit dehiscence zone and valve margin, respectively. The present study was envisaged to isolate the orthologs of SHPs and FUL from local canola “Pakola” and “Punjab Sarsoon 3”, and to study expression patterns of their transcripts. Morphological data revealed significant diference between pod wall thicknesses, seeds per pod and pod length between the two cultivars. PCR amplification and sequencing revealed that two products namely BnSHP1-like and BnSHP2-like could be identified. The sequence analysis of BnSHP1-like and BnSHP2like demonstrated that these genes are 747 bp and 735 bp in size, respectively. The nucleotide alignments revealed 98% identity of BnSHP1-like and BnSHP2-like with BnSHP1 and BnSHP2 sequences. The sequence homology was estimated to be 95 and 96% at amino acid level for BnSHP1-like and BnSHP2-like genes, respectively. The phylogenetic reconstruction of SHP1 and SHP2 homologs from other species conglomerated BnSHP1-like and BnSHP2-like into their respective clades. Semi-quantitative RT-PCR revealed overlapping expression of both the BnSHP1-like and BnSHP2-like transcripts in flower and siliques while no expression in the leaf tissues was observed. A strong expression for FUL gene was detectable in mature silique and silique from upper portion of plant as compared to other tissues of “Pakola” and “Punjab Sarsoon 3”. Our results flaunt basic gene expression information about shattering genes for developing genome edited plants to prevent yield losses in canola in future.