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High Performance Liquid Chromatographic Method for Simultaneous Determination of Alprazolam with Antihistamines in Bulk Drug, Pharmaceutical Formulation and Human Serum.

Author(s)
S. N.ALI Department of Chemistry, NED University of Engineering and Technology,Karachi, Pakistan
A. QAYOOM Department of Chemistry, NED University of Engineering and Technology,Karachi, Pakistan
S. IQBAL Department of Chemistry, Jinnah Govt. College Nazimabad Karachi, Karachi, Pakistan
N. NAZ Department of Chemistry, NED University of Engineering and Technology,Karachi, Pakistan
I. MEMON Department of Chemical Engineering, NED University of Engineering and Technology, Karachi, Pakistan
Abstract
A high performance liquid chromatographic method with UV detection for simultaneous quantification of alprazolam with antihistamine was developed and validated following the ICH guidelines. Separation was achieved on Shimadzu Shim-pack CLC-ODS (M) 25 M column employing mobile phase 80:20methanol-water with pH 3.5 at 230 nm adjusting flow rate 1.0 mL min-1. The linear concentration ranges were 0.2-12.5 µg mL-1 for alprazolam and 0.4-25 µg mL-1 for antihistamines with correlation coefficient greater than 0.998 and detection limits 2.85, 6.92, 10.56, 4.9 ng mL-1 for promethazine, levocetirizine, alprazolam, and loratadine respectively. Validation showed RSD < 2% and 98.05-101.89% recovery. Proposed method was successfully applied for quantification of studied drugs in pharmaceutical formulations without excipients interference and also in human serum.
Publication Details
Page(s) 7-12
DOI DOI not available
Published Journal: Sindh University Research Journal, Volume: 49, Issue: 1, Year: 2017

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Keywords
HPLC, method validation, antihistamine, pharmaceutical formulation
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