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Development and validation of reversed-phase high performance liquid chromatographic method for analysis of cephradine in human plasma samples.,

Author(s)
Mahmood Ahmad Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan
Muhammad Usman Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan
Asadullah Madni Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan
Naveed Akhtar Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan
Nayab Khalid Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan
Waheed Asghar Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur, Pakistan
Sattar Bakhsh Faculty of Pharmacy, Gomal University, Dera Ismail Khan, Pakistan
Abstract
An HPLC method with high precision, accuracy and selectivity was developed and validated for the assessment of cephradine in human plasma samples. The extraction procedure was simple and accurate with single step followed by direct injection of sample into HPLC system. The extracted cephradine in spiked human plasma was separated and quantitated using reversed phase Cis column and UV detection wavelength of 254 nm. The optimized mobile phase of new composition of 0.05 M potassium dihydrogen phosphate (pH 3.4)-acetonitrile (88:12) was pumped at an optimum flow rate of 1 mL.min-1. The method resulted linearity in the concentration range 0.15-20 Pgg.mL-1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.05 and 0.150 pg.mL-1, respectively. The accuracy of method was 98.68 %. This method can `be applied for bioequivalence studies and therapeutic drug monitoring as well as for the routine analysis of cephradine.
Publication Details
Page(s) 58-63
DOI DOI not available
Published Journal: Journal of Chemical Society of Pakistan, Volume: 32, Issue: 1, Year: 2010

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