Pre and post apheresis platelet CD markers’ evaluation using flow cytometry.

Saleh Abdullah; Qamar Ayub; Muhammad Saboor; Moinuddin

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Pre and post apheresis platelet CD markers’ evaluation using flow cytometry.

Author(s)

Saleh Abdullah Hematology and Blood Transfusion, Faculty of Applied Medical Sciences, Jazan University, Jazan, Saudi Arabia

Qamar Ayub Baqai Institute of Medical Technology, Baqai Medical University, Karachi, Pakistan

Muhammad Saboor Baqai Institute of Hematology, Director, Baqai Institute of Medical Technology, Baqai Medical University, Karachi, Pakistan

Moinuddin Baqai Institute of Hematology, Baqai Medical University, Karachi, Pakistan

Abstract

Objective: To evaluate the effect of apheresis procedure on platelet`s activation dependent glycoproteins` expression on their surface. Methods: Thisstudy was conducted between June 2012 and June 2014, and comprised blood and plateletsamples. Two samples were collected i.E. venous blood sample and apheresed platelet sample from the same donor. Platelet cluster of differentiation markers (41, 61, 62p and 63) were analysed within 2 hours of sample collection using flow cytometry. SPSS 20 was used for data analysis. Results: A total of 100 donors were recruited in this study. Cluster of differentiation (CD) markers` expression of 100 pre-apheresis and 100 platelet apheresis samples was compared after the completion of platelet apheresis procedure. CD 41 and 61 showed no significant difference between pre- and postapheresis platelets; (p=0.447 and 0.712, respectively). CD 62p positivity of pre-apheresis platelets (9.57±5.88%), and post-apheresis platelets (55.57±24.59%) showed statistically highly significant difference (p

Publication Details

Page(s) 1440-1443

DOI DOI not available

Published Journal: Journal of Pakistan Medical Association, Volume: 66, Issue: 11, Year: 2016

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