OBJECTIVE: To assess physician`s knowledge regarding Dengue Haemorrhagic fever. DESIGN: Descriptive cross sectional study. PLACE AND DURATION OF STUDY: Government and private hospitals and clinics in Karachi from April 2011 to Nov, 2011. METHODOLOGY: An appropriate questionnaire regarding origin, transmission, symptoms, treatment, prevention and vaccination for dengue fever was prepared and was filled by asking questions from two hundred physicians, after conducting approval for this study from the institutional review board of Dow University of Health Sciences, Karachi. RESULTS: The results indicate that 100% of the doctors were aware about Dengue viral infection but lack knowledge about its diagnosis (72%) and management, while 50% of doctors want to isolate the patient. CONCLUSION: The results concluded that more knowledge and training should be provided to doctors regarding dengue fever.