Eco-genetics and random amplified polymorphic DNA (RAPD).

Shahida Javed; Muhammad Saleem; Muhammad Siddique; Amber Chayyan

This paper describes the efficiency of RAPD technique to assess the genetic diversity of Sordaria fimicola in response to environmental exposure. Three strains were obtained from divergent microscale environments, harsh South facing slopes (SFS) and mild North facing slopes (NFS) of evolution canyon (EC). The dominant marker RAPD primed the whole genome of filamentous fungi. The fungus 1 primer show high level of polymorphism (69%). Total amplified fragments were 401 out of which 247 were polymorphic bands. Band size was between 20 to 2000 base pairs (bp). The 71 polymorphic bands scored in (strain S3 and its progeny), 66 (strain N5 and its progeny), 110 (strain Sw 92.1 and its progeny). Sixty seven percent polymorphism was observed in strain S3. Genome of Sordaria fimicola in this study was dug out 37.06 kb with five random markers (RAPD). The individuals showed heritable differences (polymorphism) within strains on South Facing Slope (SFS) in response to environmental agents. The phylogenetic tree derived on the basis of the degree of similarity reflected the genetic relationship among strains and their progeny. The hierarchical analysis was used to compare inter and intra specific variants. This was performed by using the unweighted pair group method (UPGM) with arithmetic average.