Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Intellan Capsule.

Aqib Zahoor; Hira Munir; Shaqfiuat Hussain; Kashifa Khanum; Salfia Naveed; Khan Usmanghani

Author(s)

Aqib Zahoor Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Hira Munir Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan

Shaqfiuat Hussain Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan

Kashifa Khanum Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Salfia Naveed Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan

Khan Usmanghani Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan; Herbion Pakistan (Pvt.) Limited, Karachi, Pakistan

Abstract

Simple, precise, cost-eefctive and accurate high performance liquid chromatographic method for the determination of vinpocetine in API (active pharmaceutical ingredient) and Intellan Capsule formulation has been developed and validated. Chromatography was carried out at ambient temperature on a prepacked Purospher Star, (5 mm, 250 x 4.6 mm) and Hibar 2504.6 columns with the isocratic mobile phase of acetonitrile: Ammonium acetate (55:45 v/v) adjusting pH to 2.8. The UV detection was carried at 280 nm. The results obtained showed good agreement with the declared contents. Vinpocetine detected in less than 25 mins with good resolution and minimal tailing and without interference of excepients. The method was linear in the range of 5-100 µgmL-1 with a correlation co-eficient 0.999 (inter and intraday CV<2 .0%). The recovery was 99-102%. The method was validated according to ICH guidelines and the acceptance criteria for accuracy, precision, linearity, specificity and system suitability were met in all cases. The proposed method can be used for quantitative determination of Vinpocetine from API and formulations.

Publication Details

Page(s) 28-33

DOI DOI not available

Published Journal: RADS Journal of Pharmacy & Pharmaceutical Sciences, Volume: 4, Issue: 1, Year: 2016

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Keywords

and RP-HPLC, formulation

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