Article Detail — Pakistan Science Abstracts

Choose Theme

Theme 1 — Green & Gold

No Detail Found!
The Predominant Incidence of Mycoplasma Mycoides Subsp. Capri in Suspected Cases of Contagious Caprine Pleuropneumonia in Sheep and Goats of Northern Pakistan.
Author(s)
HanifUr Rahman Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar, Pakistan
Umer Saddique Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar, Pakistan
Shakoor Ahmad Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar, Pakistan
Muhammad Kamal Shah Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar, Pakistan
Said Sajjad Ali Shah Department of Animal Health, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar, Pakistan
Farhan Anwar Khan College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan
Fazli Rabbani College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan
Muhammad Asif Hussain College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan
Irshad Ahmad College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University, Mardan, Khyber Pakhtunkhwa, Pakistan
Sadeequr Rahman College of Veterinary Sciences and Animal Husbandry, Abdul Wali Khan University Mardan, Khyber Pakhtunkhwa, Pakistan
Abstract
Contagious Caprine Pleuropneumonia (CCPP), a highly contagious and economically devastating disease, is caused by Mycoplasma mycoides (MM) - a cluster of six member species. However, literature is not available regarding the causative agents of CCPP in sheep and goats in Northern Pakistan. The current study was thus aimed to identify relative abundance of members of MM in small ruminants suspected of CCPP in Northern region of Pakistan. A total of 300 samples, (150 sheep and 150 goats) were randomly collected from nasal discharge, pleural fluid and lung tissues, and streaked into Pleuropneumonia like organism (PPLO) medium for isolation and identification of Mycoplasma species. Confirmed mycoplasma isolates were subjected to species specific PCR to identify members of MM cluster. Results revealed that 79/300 (26.3%) samples were found positive for the growth of Mycoplasma. MM cluster specific PCR showed 49 (16.3%) samples were found positive, of which 34 (11.3%) were found positive for Mycoplasma mycoides subsp. capri (Mmc) and 15 (5%) were identified as Mycoplasma capricolum subsp. capripneumoniae (Mccp) by species specific PCR. Three samples were found positive both for Mmc and Mccp suggesting mixed infection. To the best of our knowledge, we report for the first time on the molecular identification of Mccp and Mmc, and on predominance of Mccp as etiological agent of CCPP in sheep and goats of Northern Pakistan. The results have implications in the epidemiology and vaccine strategy against CCPP infection in small ruminants in the Northern region of Pakistan and therefore, a comprehensive country wide surveillance should further investigate the overall prevalence of members of MM cluster in suspected cases of CCPP in small ruminants.
Publication Details
Page(s) 1995-1998
DOI 10.17582/journal.pjz/2018.50.5.sc9
Published Journal: Pakistan Journal of Zoology, Volume: 50, Issue: 5, Year: 2018
Keywords
Keywords are not available for this article.
References
and Rahman S.,T. , Ur Rahman,S. , Zhang,L. , Shahid,M. , Zhang,S.,G. , Gao,B., 2016., 50 1071 -77
01931., -
T. , Rahman,S.U. , Zhang,L. , Shahid,Han , D.,Gao , J.,S. , Ruegg,P.L. , Saddique, .,pjz/2018.50.3 -
B., 2017., 8 90144 -90163
M. , Abbas,F. , Yasinzai,M. , Nicholas,R. , Babar,S. , Ayling,R. , Attique,M. and Ahmed,Z., 2009., -
Pak. Vet, ., 29 179 -185
Awan , M.A.,Abbas , F.,Yasinzai , M.,Nicholas , R.A.,Babar , S.,Ayling , R.D.,Attique , M.A.,Ahmed , Z.,Wadood , A.,Khan , F.A., 2010.,Mol. Biol. Rep 16 16 -3406
2017., -
Hotzel , H.,Sachse , K.,Pfützner, 1996., -
XKhattak,I. , Mushtaq,M.H. , Ayaz,S. , Ali,S. , Sheed,A. , Muhammad,J. , Sohail,M.L. , Amanullah,H. , Ahmad,I.,UrRahman,S., 2018., 49 00176 -1135
Exp. Med. Biol., ., 1057 111 -126
A. , Zendulkova,D. and Pospíšil,Z., 2001.,org/10 -
ActaVet,L. , Perrier,X. and Thiaucourt,F., 2007., 7 403 -412
Int. J.System, ., 57 2247 -2258
R. , Ayling,R. and McAuliffe,L., 2008., 64918 -
OIE, 2014.Contagious caprine pleuropneumonia, -
.OIE terrestrial manual,Chapter 2 -
Sadique , U.,Chaudhry , Z.,Younas , M.,Anjum , A.,Hassan , Z.,Idrees , M.,Mushtaq , M.,Sajid , A.,Sabtain , S.,Anim, 2012., 22 33 -37
Samiullah , S., 2013., 58 389 -398
M.K. , Saddique,U. , Ahmad,S. , Hayat,Y. , Ur Rahman,S. , Hassan,M.F. and Ali,T., 2017.,org/10 -
SmallRumin, ., 153 118 -122
W. , Munir,R. , Khan,M.S. , Ahmad,M. , Khan,M.A. , Ijaz,M. , Shakil,M. , Iqbal,M. and Ahamd,R., 2012.,org/10 44 559 -568
Shahzad , W.,Munir , R.,Rana , M.Y.,Ahmad , R.,Khan , M.S.,Akbar , G.,Ijaz , M.,Mehmood , F., 2013., -
Trop. Anim. Hlth. Prod., ., 45 737 -742
Thiaucourt , F.,Johansson , K.,Smith , D.,Wesonga, .,org/10 -
and Yigezu , L., 2000.Contagious pleuropneumonia recent advances,In 7th International Conference on Goats, France 15 -21
Citations
Citations are not available for this document.
0

Citations

0

Downloads

43

Views

Copyright ©  PASTIC National Center, Islamabad www.pastic.gov.pk