Antioxidant activity directed isolations from Punica granatum. | Pakistan Journal of Scientific and Industrial Research (Series A: Physical Sciences) 2012

Author(s)

Shahina Naz Department of Food Science & Technology, University of Karachi, Karachi-75270, Pakistan

Rahmanullah Siddiqi Department of Food Science & Technology, University of Karachi, Karachi-75270, Pakistan

Syed Muhammad Ghufran Saeed Department of Food Science & Technology, University of Karachi, Karachi-75270, Pakistan

Syed Asad Sayeed Department of Food Science & Technology, University of Karachi, Karachi-75270, Pakistan

Abstract

The extracts derived from pomegranate juice following antioxidant activity directed isolation were screened for their antioxidant activity through their ability to scavenge 2,2- diphenyl-l-picrylhydrazyl (DPPH) radicals. Only fractions which exhibited >50% DPPH scavenging effect at each step of isolation were selected for father purification and their ability to reduce peroxide formation (peroxide value) in heated com oil. Phytochemical analysis of the pure compounds finally obtained, revealed the presence of pelargonidin-3- galactose (Pg-3-galactose), cyanidin-3-glucose (Cy-3-Glucose), gallic acid, quercetin and myricetin in the fractions exhibiting >50% DPPH scavenging potential. The order of antioxidant activity of these pure compounds by DPPH method was found to be gallic acid> quercetin> myricetin> Cy-3-galactose> Pg-3-Glucose while order with respect to reduction in peroxide value (PV) was the reverse of DPPH.

Publication Details

Page(s) 14-20

DOI DOI not available

Published Journal: Pakistan Journal of Scientific and Industrial Research (Series A: Physical Sciences), Volume: 55, Issue: 1, Year: 2012

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