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 Modified picrate method for determination of cyanide in blood
Author(s)
Muhammad Avais Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan
Muhammad Sarwar Khan Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan
Muhammad Arif Khan Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan
Kamran Ashraf Department of Parasitology, University of Veterinary & Animal Sciences, Lahore, Pakistan
Amar Nasir Department of Clinical Medicine and Surgery, University of Veterinary & Animal Sciences, Lahore, Pakistan
Masood Rabbani University Diagnostic Laboratory, University of Veterinary & Animal Sciences, Lahore, Pakistan
Abu Saeed Hashmi Institute of Biochemistry and Biotechnology, University of Veterinary & Animal Sciences, Lahore, Pakistan
Abstract
Cyanide (CNÏ) is widely distributed in the ecosystem and has been associated with toxic effects in humans and animals. Most outbreaks of CNÏ poisoning in animals result from ingestion of plants containing cyanogenic glycosides. Various analytical techniques for estimating cyanide in blood are available. A simple picrate method was developed to determine blood CNÏ in goats. This assay is a modification of commonly available methods using picrate paper and those using Conway diffusion cells. Cyanide in blood was measured during and after IV administration of KCN at 0.6 mg/min for 1 h. Blood CNÏ levels in rabbits were determined after oral administration of KCN for 10, 20, 30 and 40 days. The CNÏ concentration in blood of goats was time-dependent and continued rising during infusion followed by gradual decline after infusion stopped. A calibration curve set by dissolving various concentrations of KCN in distilled water showed a linear relationship between CNÏ concentration and absorbance (R=0.995) ranging from 0.3-120 mg CNÏ/L. Blood CNÏ levels in rabbits showed time-dependent increase with maximum concentration (1.34 mg/L) at 40 days. This is a simple and inexpensive tool for the determination of blood CNÏ in the laboratory and under field conditions as well.
Publication Details
Page(s) 149-153
DOI DOI not available
Published Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 24, Issue: 2, Year: 2011
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