Antifungal Activity of a Chemically Induced Mutant Variant of Xanthoria Parietina. | Biologia 2017

Author(s)

Hafiza Nazish Hameed Institute of Industrial Biotechnology (IIB), GC University Lahore, Pakistan

Sikander Ali Institute of Industrial Biotechnology (IIB), GC University Lahore, Pakistan

BEENISH HASSAN Institute of Industrial Biotechnology (IIB), GC University Lahore, Pakistan

Abstract

In the present study, parietin was extracted from wild type and mutant strain of lichen Xanthoria parietina. Six different solvents (acetone, ethanol, methanol, hexane, chloroform and distilled water) were used to extract parietin. The parietin activity was found to be maximal when the extraction was carried out with 3 ml of methanol at 30ºC for 1 h. All the extracts were further proceeded to test the antifungal activity against different fungal species i.e., Aspergillus niger, Aspergillus oryzae, Acremonium chrysogenum, Trichoderma harz anium and Rhizopus spp. It was observed that A. niger and A. oryzae are most sensitive to X. parietina. However, Rhizopus spp. showed resistance against lichen extracts except ethanol. The methanol extract of EMS mutated strain proved to be more effective to inhibit the growth of A. niger. Moreover, 62.5% stronger antifungal activity was observed within the treated sample as compared to untreated sample. HPLC analysis also represented more than 90% purity of methanol extract with some trace elements. The results showed that studied lichen species possess strong antifungal activity.

Publication Details

Page(s) 79-85

DOI DOI not available

Published Journal: Biologia, Volume: 63, Issue: 1, Year: 2017

Keywords

Antifungal Xanthoria parietina chemical mutagen parietin secondary metabolite

References

Agarwal , S.,Singh , S.S.,Verma , S.,Kumar , S.,,2000,Antifungal activity of anthraquinone derivatives from Rheum emodin,J. Ethnopharm.,72,43,46

Backorova , M.,Backor , M.,Mikes , J.,Jendzelovsky , R.,P.,,2011,Variable responses of different human cancer cells to the lichen compounds parietin, atranorin, usnic acid and gyrophoric acid,Toxicol,25,37,44

Cornejo , A.,Salgado , F.,Caballero , J.,Vargas , R.,Simirgiotis , M.,Areche , C.,,2016,Secondary metabolites in Ramalina terebrata detected by UHPLC/ESI/MS/MS and identification of parietin as tau protein inhibitor,Int. J. Mol. Sci,17,1303,1316

Edwards , H. G.,Newton , E. M.,Williams , D. D. W.,Coombes , S. R.,,2003,Molecular spectroscopic studies of lichen substances 1: parietin and emodin,J. Mol. Struc,648,49,59

Gauslaa , Y.,McEvoy , M.,,2005,Seasonal changes in solar radiation drive acclimation of the sun-screening compound parietin in the lichen Xanthoria parietina,Basic. App. Ecol.,6,75,82

Huneck , S.,YoshimuraI.,,1996,,,,11,

Lucking , R.,,2014,A field guide to California lichens,,117,427,428

Manojlovic , N.,Solujic , S.,Sukdolak , S.,Krstic , L.,,2000,Isolation and antimicrobial activity of anthraquinones from some species of the lichen genus Xanthoria,J. Ser. Chem. Soc.,65,555,560

Nash , T. H.,,2008,,,315,,

Rankovic , B. R.,Kosanic , M. M.,Stanojkovic , T. P.,,2011,Antioxidant, antimicrobial and anticancer activity of the lichens Cladonia furcata, Lecanora atra and Lecanora muralis,BMC Complement. Altern. Med,11,89,97

Sisodia , R.,Verma , S.,Rani , A.,Dureja , P.,,2013,Antibacterial and antioxidant activity of lichen species Ramalina roesleri,Nat. Prod. Res.,27,2235,2239

Sloczynska , K.,Powroznik , B.,Elzbieta , P.,Waszkielewicz , A. M.,,2014,Antimutagenic compounds and their possible mechanisms of action,J. Appl. Genet,55,273,285

Snedecor , G. W.,Cochran , W. G.,,1980,,,,32,

Solhaug , K. A.,Gauslaa , Y.,,1996,Parietin, a photoprotective secondary product of the lichen Xanthoria parietina,,108,412,418

Volff , J. N.,Altenbuchner , J.,,1998,Genetic instability of the Streptomyces chromosome,Mol. Microbiol,27,239,246

Yoshimura , I.,Kinoshita , Y.,Yamamoto , Y.,Huneck , S.,Yamada , Y.,,1994,Analysis of secondary metabolites from lichen by high performance liquid chromatography with a photodiode array detector,Phytochem. Anal.,5,197,205

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