Using standard analytical methods and the atomic absorption technique, certain physico-chemical parameters and trace metal levels are estimated for the water samples from holy water Zamzam, collected from Mecca, Saudi Arabia, during Haj period from 1983 through 1985. The estimated parameter include pH chloride, sulphate hydrogen carbonate hardness (CaCO3) conductance, nitrate, Total Dissolved Solids (TDS) and silica. At optimized atomic absorption conditions the trace metal analysis is conducted for sodium, potassium, iron zinc calcium, magnesium, lead, cadmium, strontium, copper, cobalt, manganese mercury and chromium. Averaged results for replicate measurements are reported at ±2S confidence level. The data obtained are compared with those for local, non-treated surface potable water, hot spring water and sea water. The interrelationship is examined from seasonal and/or regional viewpoints.