Bacterial antimicrobial resistance (BAMR) seems to pose the greatest threat topublic health, food safety, and agriculture in this century. The development ofnovel, efficient antimicrobial agents to combat bacterial infections has become aglobal issue. Silver nanoparticles (Ag NPs) appeared as a feasible alternative toantibiotics. However, Ag NPs face cost, toxicity, and aggregation issues whichlimit their antibacterial activity. This work aims to stabilize Ag NPs withenhanced antimicrobial activity at comparatively lower Ag concentrations toprevent bacterial infections. For this purpose, the Ag core was covered withnanodiamonds (NDs). Ag-NDs composite have been synthesized bymicroplasma technique. TEM analysis confirmed the presence of both Ag andNDs in the Ag-NDs composite. A particle size (~19 nm) was reported for Ag-NDs at the highest concentration as compared to Ag NPs (~3 nm). Theconduction band of the diamond acted as an extremely strong reducing agent forAg NPs. The large surface area of NDs stabilized the Ag NPs. A redshift (~400nm-406 nm) in UV-visible spectra of the Ag-NDs composite indicated theformation of bigger-sized Ag NPs after incorporating NDs. XRD and LIBSanalysis verified the increase in intensity of Ag-NPs by increasing NDconcentration. The presence of functional groups including OH, CH, andAg/Ag2O was confirmed by FTIR. Bacterial inhibition growth appeared to be adose-dependent process. The minimum inhibition concentration value of Ag-NDs composite at the highest NDs concentration against E. coli (~ 0.69 µg/ml)and S. aureus (~44 µg/ml). This is the first study to report the smallest MIC forE. coli (<1 µg/ml). Ag-ND composites emerged to be more efficient than AgNPs and preferred to be used against BAMR. The enhanced antibacterial activityof the Ag-NDs composite makes it a potential candidate for antibiotics, foodproducts, and pesticides.