Pakistan grapples with a massive problem of massive municipal solid waste(MSW) management. The abundance of organic solid waste contributes toenvironmental degradation. Thus, effective strategies for MSW management areessential. We report the extraction of cellulose from waste cardboard and itsconversion to cellulose nanofibres (NC), with high surface area, thermal andmechanical stability, and biocompatible nature. XRD confirmed the extractednanocellulose as cellulose type I with 61% degree of crystallinity. The size andzeta potential of NC was found to be 733 nm and 4.67 mV, respectively. Since thedispersion of NC poses a problem but is necessary for the purpose of application, apyridinium based ionic liquid [C4Py] [Cl] (PyIL) was synthesized as the greensolvent. The ionic liquid was characterized by nuclear magnetic resonance(NMR), FTIR and ultraviolet visible (UV-Vis) spectroscopy. The dynamicviscosity and density of the PyIL was found to be 560 mPas-s and 0.895 g/cm3 atroom temperature. Anti-microbial activity of NC, PyIL and cellulose dissolvedionic liquid (CIL) was investigated by broth dilution method against grampositive strains (Bacillus Subtilis and S. Aureus) and gram negative strains (K.Pneumoniae and E.Coli). Antioxidant activities of these compounds wereanalyzed using DPPH and FRAP assays. The best antioxidant activity was shownby CIL with 24.98% radical scavenging activity (RSA) against DPPH and549.92 µg/mL of ascorbic acid equivalent per mg of sample. Excellenthemolytic inhibition activity was shown by CIL with a highest value of 150%.Results indicate that the anti-microbial, anti-oxidant and hemolytic inhibitionactivities of NC were greatly enhanced upon dissolution in PyIL.