BOT-1929: Eco-Friendly Synthesis of Iron Oxide Nanoparticles using Opuntia dillenii, Characterization and Bioactivities | 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025 2025

Author(s)

Madiha Imtiaz Department of Botany, Bacha Khan University,Charsadda, 24420, Khyber Pakhtunkhwa,Pakistan

Javed Iqbal Department of Botany, Bacha Khan University,Charsadda, 24420, Khyber Pakhtunkhwa,Pakistan

Banzeer Ahsan Abbasi Department of Botany, Rawalpindi Women University,6th Road, Satellite Town, Rawalpindi 46300,Pakistan

Farishta Zarshan Department of Botany, Bacha Khan University,Charsadda, 24420, Khyber Pakhtunkhwa,Pakistan

Abstract

In the current research work, bioactive components present in Opuntia dillenii fruit extract were utilized for the synthesis of IONPs using iron acetate as precursor salt. The newly formed IONPs were extensively characterized for their physiochemical properties using different analytical tools; UV-Vis spectroscopy, SEM, EDX, FT-IR, and XRD. The crystalline nature of IONPs was confirmed by XRD analysis and the average size was calculated 27.7 nm. Further, in vitro studies were performed to reveal biological potentials. Antibacterial assay of biosynthesized IONPs was performed against different bacterial strains (Staphylococcus saprophyticus, Enterococcus faecalis and Rhodococcus jostii) and zone of inhibition (ZOI) were measured and MIC values for all strains was reported as 31.25 µg/mL. The IONPs showed antifungal activity against different fungal strains (Aspergillus niger, Aspergillus flavus, and Candida albicans). The MIC value for Aspergillus niger and Candida albicans was reported as 100 µg/mL and described as the most susceptible strains while Aspergillus flavus was reported as the most resistant strain MIC: 300 µg/mL. To evaluate the cytotoxicity assay against newly hatched nauplii of brine shrimps, Vincristine sulfate served as positive control. The results for IONPs was recorded as 41.73 µg/mL while 4.011 µg/mL was reported for positive control vincristine sulphate. Overall, the current study has demonstrated the utilization of Opuntia dillenii fruit extract for IONPs fabrication by designing an eco-friendly and forthright alternative to traditional approaches for IONPs synthesis.

Publication Details

Page(s) 61-61

DOI DOI not available

Published Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025

Keywords

cytotoxicity antibacterial Antifungal Iron oxide nanoparticles Opuntia dillenii

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