This observational study aimed to evaluate the use of a single portable device to assess the non-invasive tear break-up time (NITBUT), tear meniscus height (TMH), and lipid layer patterns (LLP) in young females with refractive errors (REs). The study was conducted at the College of Applied Medical Science (Female campus), Riyadh, Saudi Arabia between January 5, 2021 to May 15, 2021. Forty young females, with mean age of 23.0± 4.3 years with REs (-2.53 ± 2.05 D) and 40 females, mean age 23.8± 4.5 years with healthy eyes were recruited. The tests were administered in the following order: Ocular Surface Disease Index (OSDI), followed by NITBUT, TMH, and LLP. Signicfiant differences (via Mann-Whitney U test) were noted in the median ocular surface disease index (OSDI; p?0.001), NITBUT (p=0.035), TMH (p=0.009), and LLP (p?0.001) scores between the study and control groups. Females with REs have signicfiantly lower lipid layer, TMH, and NITBUT scores than those with healthy eyes.