Cotton provides a living for 25 million Pakistanis and a major raw material for textile sector which accounts for 75 % of the fiber mix in textile industry products. Fiber yield and quality are very crucial parameters for a cotton breeding program. Five varieties namely FH-414, FH-415, FH-490, FH-492, and FH-ANMOL were used as lines and crossed with three testers namely NIAB- SANAM, CIM-602, and EYE-111 during the year 2019-20. Data was recorded for plant height (PH), number of monopodial branches (MB), number of sympodial branches (SB), number of bolls/plant (BP), boll weight (BW ), ginning out turn (GOT), fiber fineness (FF), fiber length (FL), fiber strength (FS) and seed cotton yield (SCY ). FH414 proved to have very good GCA for BW and FL. FH-415 showed good GCA for FL and monopodial branches. Similarly, FH-490 for GOT and FF, FH-492 for SB, BW, BP and SCY. FH-ANMOL proved best combiner for, SB, BP, and SCY. CIM-602 was good combiner for BP, SB, BW, GOT, FS and SCY. EYE-111 found to be a good combiner for BP, GOT, and FF. The hybrid FH-415 × CIM-602 showed good SCA for MB and FH-ANMOL × EYE-111 for SB. For bolls/plant (BP) FH-492 × EYE-111, for boll weight (BW ) FH-ANMOL × EYE-111, for GOT FH-415 × EYE-111, for fiber fineness (FF) FH-414 × EYE-111, for fiber length (FL) FH-92 × NIAB-SANAM, for fiber strength (FS) FH-414 × CIM-602 and for seed cotton yield (SCY ) FH-492 × EYE-111 proved to be very good hybrid. These results indicated that these lines and testers have desired variability to exploit in the traits which were investigated.