Global studies carried out as case-control and meta-analysis studies confirmed all forms of associations in FABP2 gene within obesity. Saudi Arabia is recording as one of the high prevalence of obesity in the gulf region and this studies principle objective is to investigate the role of A54T genotype in FABP2 gene in obesity subject. This work was designed by involving 102 obesity confirmed cases and 102 controls. PCR analysis was carried out after extracting the genomic DNA from all the subjects and then RFLP analysis was performed. Anthropmetric measurements and lipid profile showed the higher frequency among obesity. T allele, TT genotype and dominant model showed the positive association (p=0.05). Anova analysis revealed the higher frequency of TT genotypes among WC, weight and BMI (p=0.05). This study confirms T allele and TT genotype including dominant model showed the positive association with A54T genotype in FABP2 gene in the obese subjects.