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Metagenomic Analysis and Identification of Epizootic Strains of the Causative Agent of Infectious BovineKeratoconjunctivitis in Kazakhstan
Author(s)
Rano Sattarova Laboratory of Bacteriology, LLP “Kazakh Scientific Research Veterinary Institute”,Almaty,Kazakhstan
Kuandyk Shynybaev Laboratory of Bacteriology, LLP “Kazakh Scientific Research Veterinary Institute”,Almaty,Kazakhstan
Flyura Bakiyeva Laboratory of Bacteriology, LLP “Kazakh Scientific Research Veterinary Institute”,Almaty,Kazakhstan
Vitaliy Strochkov Laboratory of Bacteriology, LLP “Kazakh Scientific Research Veterinary Institute”,Almaty,Kazakhstan
Karlygash Boranbayeva Laboratory of Bacteriology, LLP “Kazakh Scientific Research Veterinary Institute”,Almaty,Kazakhstan
Orik Zhanserkenova Green Biotechnology and Cell Engineering Laboratory, Kazakh National Agrarian Research University,Almaty,Kazakhstan
Shinara Kassymbekova Green Biotechnology and Cell Engineering Laboratory, Kazakh National Agrarian Research University,Almaty,Kazakhstan
Akerke Ibadullayeva Green Biotechnology and Cell Engineering Laboratory, Kazakh National Agrarian Research University,Almaty,Kazakhstan
Aigerim Khamzina Green Biotechnology and Cell Engineering Laboratory, Kazakh National Agrarian Research University,Almaty,Kazakhstan
Abstract
This research aimed to identify the causative agent of infectious bovine keratoconjunctivitis (IBK) Moraxella strains and differentiate them using 16S rRNA sequencing and PCR. The 16S ribosomal RNA (16S rRNA) gene sequencing method was used to determine the microbiome. This is due to the high commonness of this gene in the genomes of all prokaryotes, which allows selecting universal primers to strengthen areas of interest and reduce the cost and time of research. PCR-RT was used for the identification of Moraxella strains. In this study, we identified the causative agents Moraxella bovis and Moraxella bovoculi based on the metagenomics analysis of infected cattle in the Republic of Kazakhstan. We visualized the obtained data using Krona's diagram and EDGE bioinformatics software. We differentiated M. bovis and M. bovoculi from other cultures of Moraxella spp. using PCR-RT. For the first time in Kazakhstan, strains that cause IBK were identified using metagenomic analysis. This raises the diagnosis of IBK, a widespread infectious cattle disease, to a genetic level. Our research will serve as the foundation for the further scientific study of the accompanying microorganisms, followed by recommendations for herd welfare and the prevention of economic losses associated with the death and therapeutic manipulations of sick animals.
Publication Details
Page(s) 822-831
DOI 10.47278/journal.ijvs/2023.071
Published Journal: International Journal of Veterinary Science, Volume: 12, Issue: 6, Year: 2023
Keywords
Cattle Moraxella Pink Eye 16S RNA Sequencing PCR Real Time
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