The purpose of this study was to evaluate nutritional content of tofu waste mixed with carrot juice and fermented with Saccharomyces spp. (TWCF), so that it has potential as a feed supplement that is rich in ß-carotene, high in protein and lysine, and contains probiotics. The laying hens (n=192, 70 weeks old) were randomly allotted into 4 diets groups. The hens were fed diets i) without TWCF as a control diets (TF0), ii) supplemented with 2% TWCF (B); iii) supplemented with 4% TWCF (C); and iv) supplemented with 6% TWCF (D). Inclusion of TWCF in diets increased nutrient digestibility (DM, OM, and CP) significantly (P=0.05). Fermentation of TWC with YC (TWCF) can increase (P=0.05) nutrient of TWC especially: CP, Lysine, and BC. Inclusion up to 2% TWCF can be used in laying hen diets to improve (P=0.05) feed digestibility (DM, OM, CP), egg production, eggshell thickness, yolk color, BC, and yolk cholesterol. It is concluded that dietary supplementation of laying hens with TWCF can increase hen-day production, egg shell thickness, BC, and egg yolk color.