Genotype by environment interaction (GEI) imposes an influence onthe yield stability of a genotype tested under erratic environments. The present experiment was carried out at six sites viz; Multan, Faisalabad, Khanpur, Bahawalpur, Vehari and Sahiwal in the Punjab province during Kharif 2020-21. The main objectives of the study were to quantifythe GEI component of variation and to assess the yield stability of recently bred upland cotton strains across test environments. Twenty-one cotton strains were sown according to a randomized complete block design (RCBD) with three replications. Additive main efects and multiplicative interaction (AMMI) analysis illustrated that additive efects of genotypes, environments and multiplicative efects (GEI) were significant at (p<0.01). Test sites accounted for the biggest share (74.1%) of the variability, followed by GEI (17.2%) and strains (8.7%), respectively. The first four interaction principal components (IPC) were significant at (p<0.01) wherein the first two IPCs captured (68.4%) of the GEI cumulatively. Strain ST06 (FH-492) won four environs and was declared the overall winner of this trial. The Vehari location was the best location for cotton multi-environment trials (MET) due to the bearing of minimum IPC-1 score. The correlation coeficient between strains in IPC1 was (0.81) and sites in PC1 was (0.76). Strain ST08 (BH-224) bears a yield edge of (84.5%) over general winner strain ST (06) FH-492 at the site of Multan due to micro adaptations. Genotype selection index (GSI: A non-parametric approach to determine yield stability) discriminated ST02 (WEAL-AG-10), ST05 (MNH-1050), ST09 (VH-418) and ST19 (UAM-20) as stable yielder strains bearing the lowest GSI value (11). These strains are important in boosting cotton production hence, their release for general cultivation may be sought from the authorized forum.