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Synthesis of selenium nanoparticles using root extract of zingiber officinale and evaluation of their antibacterial activity against escherichia coli
Author(s)
Ayesha Qaisar Department of Zoology,Wildlife and Fisheries
Syeda Maryam Hussain Department of Livestock Production and Management.
Muhammad Zishan Ahmad Department of Veterinary Pathology
Naveed Iqbal Raja Department of Botany
Murtaz Ul Hassan Department of Parasitology and Microbiology
Muhammad Shoaib Department of Parasitology and Microbiology
Saif Ur Rehman Department of Parasitology and Microbiology
Aayesha Riaz Department of Parasitology and Microbiology
Zaib Ur Rehman Department of Poultry Science, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Ali Ahmad Microbiology Section, Department of Pathobiology, Narowal Campus, University of Veterinary & Animal Sciences, Lahore, Pakistan
Zahid Manzoor Department of Parasitology and Microbiology.
Abstract
Poultry industry in Pakistan is a thriving agricultural sector, meeting the demand for affordable meat and serving as a significant protein source. However, disease outbreaks and the widespread use of antibiotics have contributed to the emergence of antibacterial resistance, posing a global concern for healthcare authorities. Responsible antibiotic use, along with alternative strategies like nanoparticlesis crucial to mitigate resistance development and safeguard public health in the poultry industry. This study aimed to assess the potential of selenium nanoparticles (SeNPs), produced through phytosynthesis using the root extract of Zingiber officinale, in inhibiting the growth of pathogenic bacteria, specifically Escherichia coli (E.coli). Various characterization techniques such as Ultraviolet–visible spectroscopy (UV-Spectroscopy), X-ray diffraction (XRD), Energy Dispersive XRay Analysis (EDX), Fourier Transform Infrared Spectroscopy (FTIR), and Scanning Electron Microscopy (SEM) were employed to analyze the synthesized SeNPs. The results indicated that the nanoparticles were approximately 80-100 nm in size, exhibited a nearly spherical shape, and possessed functional groups as well as stability properties as revealed by FTIR analysis. The antibacterial efficacy of SeNPs against E.coli was evaluated using the disc diffusion method at various concentrations. The findings demonstrated that the phytosynthesizedSeNPs effectively inhibited the growth of E.coli, exhibiting the largest zone of inhibition with a diameter of 22mm and the smallest zone of inhibition with a diameter of 9.5mm. These results suggest that phytosynthesized selenium nanoparticles hold promise as an alternative to conventional antimicrobial agents for managing colibacillosis
Publication Details
Page(s) 106-106
DOI DOI not available
Published Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023
Keywords
Escherichia coli Zingiber officinale Colibacillosis Selenium nanoparticles
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