Burn wound healing is a complex and multistep process. Proper and complete healing requires adequate moisture, microbial prevention, exudates absorption, and scab formation prevention at the wound bed. Aqua-gel is a suitable carrier system that possess all above mention characteristics for burn wound healing. In this study, Carbopol 934 and polyethylene glycol based aqua-gel was designed by incorporating 2% Calotropis gigantea extract. Various formulations were developed and F2 was considered Optimized while it was subjected to FTIR analysis, Scanning electron microscope, HPLC content determination, rheological behaviors, invitro release & release kinetics, pH and swelling behaviours. The results demonstrated that Calotropis gigantea loaded aqua-gel has great potential to increase the burn wound healing rate as compared to control group