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Indigenous Strain Aspergillus Niger PHY215 Isolated from Livestock Farm Soil: Optimization of Culture Condition for Phytase Production
Author(s)
Zahid Manzoor Department of Parasitology and Microbiology, UVAS, Lahore, Pakistan
Aftab Ahmad Anjum Institute of Microbiology, UVAS, Lahore, Pakistan
Aayesha Riaz Department of Parasitology and Microbiology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Syeda Maryam Hussain Department of Livestock Production and Management, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Saif Ur Rehman Department of Parasitology and Microbiology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Zaib Ur Rehman Department of Poultry Science, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Ayesha Qaisar Department of Zoology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
Ali Ahmad Microbiology Section, Department of Pathobiology, Narowal Campus, University of Veterinary & Animal Sciences, Lahore, Pakistan
Abstract
Phytase is an enzyme that belongs to the phosphatase group and is responsible for catalyzing the hydrolysis of phytic acid, which results in the release of inorganic phosphorus in a usable form. This study was undertaken with main objectives of meeting the growing industrial demands of phytase by standardization of local production at low cost and here we focused on optimization of culture conditions for phytase production by indigenous strain of Aspergillus niger PHY215 isolated from soil of livestock farms. Best phytase producing fungi was selected depending on size of zone of hydrolysis on plate of phytase screening medium agar and identified as A. niger PHY215 by morphological methods. Using one variable approach, culture conditions were optimized for maximum production of phytase by A. niger PHY215. Temperature, pH, substrate type and concentration were optimized in this study using submerged fermentation. Results revealed that maximum phytase production 2.45 U along with 9.81 g/L biomass of A. singer PHY215 was observed at temperature 400C, pH 5 and 3% rice bran as substrate. Phytase activity was increased in up to 2.95-fold and the biomass matter was increased in up to 3.01-fold. Under optimized conditions agricultural byeproducts such as rice bran can be used for cost effective phytase production. Hence indigenous strain of A. niger PHY215 can be used for large scale phytase production to meet the phytase requirements of animal feed industries.
Publication Details
Page(s) 53-53
DOI DOI not available
Published Journal: Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23, Volume: 0, Issue: 0, Year: 2023
Keywords
Enzyme Livestock optimization phytase Aspergillus niger
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