The main causes of respiratory, gastrointestinal, and urinary tract infections are gramnegative bacteria from the family Enterobacteriaceae. The current study was carried out to assess the presence of Enterobacteriaceae in the fecal samples isolated from the migratory birds in Khyber Pakhtunkhwa Pakistan. The fecal samples were collected from common pochard (Aythya ferina), gadwall (Mareca strepera), pintail (Anas acuta), red-head americana (Aythya americana) and teal ducks (Anas crecca) etc. The fecal samples post serial dilution were cultured twice on nutrient agar media to obtain a pure culture. Subsequently, the gram-negative bacterial colonies were identified through colony morphology on differential media. For further identification of bacterial isolates, Gram-staining and biochemical characterization was carried out. The analysis of the data revealed the occurrence of four species of Enterobacteriaceae viz., Escherichia coli, Proteus spp., Shigella and Salmonella. The current study concludes that migratory birds are a reservoir of gram-negative bacteria belonging to family Enterobacteriaceae. Migratory birds act as vectors for the transport of these pathogenic bacteria over long distances due to their inherent ability. They are important sentinels of infections and must be considered during management practices to curb pathogenic bacteria.