ASSVd infection transported by white flies plants constrains plants development | Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 2023

Author(s)

Zaryab Khalid Sial Department of Botany, LCWU, Lahore, Pakistan

Kashmala Department of Botany, LCWU, Lahore, Pakistan

Shabnum Shaheen Department of Botany, LCWU, Lahore, Pakistan

Riffat Siddique Sana Khalid Department of Botany, LCWU, Lahore, Pakistan

Abstract

The viroids are the smallest living infectious agents without any protein coat that are thought to only infect plants. Nucleic acid (DNA) transfer between plants is a natural phenomenon which is likely to occur in many ways. Apple skin scar viroid has broad herbaceous range of host plants. The active transmission of Apple scar skin viroid (ASSVd) and naked ssRNA species by the whitefly Trialeurodes vaporariorum (Tv) was confirmed during present study in cucumis sativis. It was matter of great interest that not only the viroidal RNAs, its DNA type was also identified from the targeted insect. The viroid transfer efficiency was enhanced with the help of Cucumis sativus Phloem protein 2 (CsPP2), This protien/ASSVd complex was stably present in the viroid infected cucumber plants, as was identified with the help of immunological Assay.As viroid-like secondary structures are found in some plant RNAs, and PP2 is known to bind and translocate several RNAs, the results have huge implications in transfer of these RNA species between plants visited by the whitefly. To our knowledge, there are no confirmed reports that demonstrate the use of host molecular markers for the purpose of detecting generic plant viroidal infection.

Publication Details

Page(s) 188-188

DOI DOI not available

Published Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords

Cucumis sativus Apple skin scar viroid hloem Protein 2 ssRNA Nucleic acid DNA

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