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Crop Traits Improvement through Genetic Modification and Genome Editing Approaches
Author(s)
Abdul Qayyum Rao CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Allah Bakhsh CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Ayesha Latif CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Saira Azam CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Aneela Yasmeen CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Naila Shahid CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Ambreen Gul CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Sana Shakoor CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Sahar Sadaqat CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Sara Ajmal CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Muhammad Awais CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Muhammad SaadBhutta CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Ramsha Ajmal CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Muhammad Saad Tahir CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Maham CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Hafiz Muhammad Zeeshan CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Sania Naeem CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
SamaviaWirk CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Muntaha Khalid CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Samran Sardar CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Tahir Rehman Samiullah CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Munim Farooq CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
M. Tariq CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
M. Usman Arif CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Farah Naz CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Saman Riaz CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Rida Hameed CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Saqib Ali CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Noman Nazik CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Zil Huma CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Muneeba Shahid CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Atif Shahzad CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Momna Muhammad CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Mehwish Imtiaz CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Ujala Nasir CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Kausar Malik CEMB, University of the Punjab,Thokar Niaz baig Lahore, Pakistan
Abstract
CEMB, being pioneer in developing insect and weedicide resistant crops, is successful in approval of its insect and weedicide resistant cotton varieties on commercial basis. To further enhance the toxin level next generation Btalong with fusion gene strategy is also addressed to cope with challenges of resistance buildup in insect pests. Advancement of technology to edit different traits of crop plant is fundamental to be utilized for improvement of major traits of plants like dealing with challenge of CLCuV in cotton, enhancing the storage life of potato, development of potato with enhanced vitamin A and development of resistance in potato against fungus and PVX, PVY. Further abiotic stress tolerant crop is the challenge of current and years to come to be dealt with genome editing is also established at CEMB. Two success stories of genome editing through CRISPR Cas9 system includes knock out of CLCuV targeting DNA A and Beta satellite akong with improved shelf life of potato through knockout of Vlnv has been discussed in current presentation. The knockout efficacy of the 72%for DNA-A, 90% of betasatellite was achieved during this study. The qPCR confirmed the successful reduction of the vial titre and the feeding assay showed 90% of mortality in the whiteflies. The study results were also validated by the change in Alpha chain, Beta Chains, and loops of the 3D protein models of native and edited proteins predicted with Alpha Fold2.The knockout efficiency and the virus inoculation assay magnificently determined the faith in using this technology for plant virus control along with its vector. The amplicon sequencing data showed maximum indel frequency for potato plant T12 (14.3%) resulting in 6.2%gene knock out and 6% frame shift. Whilefor plant B4, the maximum indel frequency of 2.0% was found which resulted in 4.4%knockout and 4%frameshift asanalyzed by Geneious. The qRTPCR data revealed that mRNA expression of VInvgenewasreduced90-99-fold in edited potato plants when compared to the non-edited control potato plant. Following coldstorage, chipsanalysis of potato esproved B4 and T12 asbest lines. Reducing sugars' analysis by titration method determined five fold reduction in percentage of reducing sugars in tubers of B4 transgenic lines as compared to the control.
Publication Details
Page(s) 6-6
DOI DOI not available
Published Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords
genetic modification Genome Editing Approaches Crop Traits
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