Wheat (Triticum aestivum) as staple food ensures around 20% global calories of human consumption. Yellow rust (Yr) pathogen Puccinia striiformis f. sp. tritici (PST) significantly reduced wheat production. The current study was conducted to identify the stripe rust virulence pattern and the prevalence of the most dominant stripe rust races in 2018-19. The virulence analysis identified 8 PST races from analyzed samples, and amongst these races, 564200 was the most frequent race, followed by 574201 and 574200. The rest of the races, including 574210, 564202, 560200, 574232 and 164200, constituted 6.66% of all the identified races. No virulence was found for Yr5, Yr10, Yr15, Yr24, Yr32, YrSp and YrTye whereas, all the isolated races/pathotypes were found virulent to genes including Yr1, Yr8, Yr9 and Yr27. The genes Yr6 and Yr17 were found susceptible against all races except race 164200 and 560200, respectively. Race 574210 found virulent to Yr43, while 574232 found virulent to Yr42 and Yr43. Yr genes avirulence/virulence information can be utilized in gene deployment and breeding programs in Pakistan.