Alpha-tocopherol is the most active form of vitamin E that exists in human plasma. Alpha tocopherol is a lipid-soluble antioxidant that is generally prepared only by photosynthetic organisms. It acts as the inhibitor of oxidative stress, defence of cell membrane, regulates platelet aggregation and maintains human health. The purpose was to investigate methods for the measurement of vitamin E in nuts and grains. This study was helpful to determine different concentrations of alpha-tocopherol. Vitamin E is a necessary fat-soluble nutrient that is mostly found in plant-based oils, nuts, cereals, seeds, fruits and vegetables. By using UV-VISIBLE spectroscopy and fourier transform infrared (FTIR) spectrometer, alpha-tocopherol levels in various edible grains and nuts from different areas of Lahore, Pakistan. The oils were extracted from samples by the Soxhlet method using n-hexane as a solvent. The concentrations of alphatocopherol in almond, walnut, cashew, pistachios, peanut, wheat, rice, barely, corn and canola seeds were 36.9, 0.5, 0.10, 0.3, 9.0, 212.2, 3.5, 1.04, 2.1 and 1.4mg/100g. The results reveal that the maximum concentration of alpha-tocopherol is present in wheat and the minimum concentration in cashew. The findings highlight the need of including edible nuts and grains in daily diet to address vitamin E insufficiency in humans by demonstrating that these foods provide adequate level of alpha-tocopherol.