Article Detail — Pakistan Science Abstracts

Choose Theme

Theme 1 — Green & Gold

No Detail Found!
CRISPR/Cas9-mediated Genome Editing in Potatoes
Author(s)
Aneela Yasmeen Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Abdul Qayyum Rao Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Saira Azam Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Ayesha Latif Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Naila Shahid Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Allah Bakhsh Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Sana Shakoor Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Sara Ajmal Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Sahar Sadaqat Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Momna Muhammad Centre of Excellence in Molecular Biology, University of the Punjab,Lahore,
Muhammad Awais Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Abdul Raouf Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Mehwish Imtiaz Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Tayyab Husnain Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Kausar Malik Centre of Excellence in Molecular Biology, University of the Punjab,Lahore, Pakistan
Abstract
CRISPR-Cas9 is a state of art technology that has enabled researchers to edit genomes. The present study was conducted to investigate the efficiency of genome editing in locally grown potato variety (AGB purple) in comparison to a diploid variety (M6). The stem internodes of 5-6 weeks old potato plants of both varieties were infected with Agrobacterium strain LBA4400 harboring CRISPR-Cas9 vectors expressing a particular guide RNA against vacuolar invertase; and Cas9 endonuclease. Both potato varieties showed encouraging regenerative response on selection media. The overall transformation efficiency of potatoes was found to be 11.7% whereas the indel% was found to be 6 for diploid and 5 for tetraploid variety via Sangers’ sequencing data. The regenerated plantlets were shifted to culture tubes and then to pots after development of proper roots and shoots. The molecular analysis confirmed the integration of cassette and expression of Cas9 in primary transformants. Realtime and Sanger’s Sequencing results concluded that the CRISPR/cas9 can be efficient tool for editing of plants that can be further used in an efficient potato breeding program.
Publication Details
Page(s) 182-182
DOI DOI not available
Published Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords
genome editing CRISPRCas9
References
References are not available for this document.
Citations
Citations are not available for this document.
0

Citations

0

Downloads

174

Views

Copyright ©  PASTIC National Center, Islamabad www.pastic.gov.pk