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A Comparative Analysis of Wound Healing Potential, Cell Viability and Morphology of Normal and Gestational Diabetic Affected Wharton's Jelly Derived Mesenchymal Stem Cells
Author(s)
Nabila Rasheed Department of Anatomy, Faculty of Basic Health Science, Ziauddin University, Karachi, Pakistan
Bushra Waseem Department of Anatomy, Faculty of Basic Health Science, Ziauddin University,Karachi, Pakistan
Kanwal Haneef Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Pakistan
Shumaila Usman Department of Research, Ziauddin University,Karachi, Pakistan
Madeeha Sadiq Department of Anatomy, Faculty of Basic Health Science, Ziauddin University,Karachi, Pakistan
Tabinda Urooj Department of Anatomy, Faculty of Basic Health Science, Ziauddin University,Karachi, Pakistan
Sarah Tarique Dr. Zafar H. Zaidi Center for Proteomics, University of Karachi, Pakistan
Dabeeran Zeha Department of Pharmacology, Faculty of Basic Health Science, Ziauddin University,Karachi, Pakistan
Abstract
Stem cells derived from Wharton’s jelly of human umbilical cord (WJMSCs) are considered as the potential therapeutic agents for regeneration and are a valuable source for stem cell banking. Current investigation shows metabolic disorders are not only responsible for genetic problems and certain disease risk factors in adult life but also have adverse effects on perinatal environment. This study was designed for the comparative analysis of viability and healing properties of GDM affected WJMSCs (GDMWJMSCs) with Normal controls (N- WJMSCs). Mesenchymal stromal cells were isolated from Wharton’s jelly of human umbilical cords from normal and gestational diabetic mothers. Cell viability was tested by trypan blue exclusion method and wound healing potential by scratch assay method. Healing of the scratch was observed at different time intervals (0h, 6h, 24h, 48h). The images were analyzed by Image J software. Morphological analysis was done under inverted microscope. Results showed that with each passaging stage the viability of GDM cells remained normal whereas Wound healing potential of GDMWJMSCs was decreased as compared to controls. Moreover, there were few morphological changes observed between the two groups. In conclusion GDM affects wound healing potential of WJMSCs and morphology of the cells to some extent whereas the viability of cells remains unaffected by GDM.
Publication Details
Page(s) 607-614
DOI 10.17582/journal.pjz/20201012081021
Published Journal: Pakistan Journal of Zoology, Volume: 54, Issue: 2, Year: 2022
Keywords
Wound healing Gestational diabetes Regenerative medicine Inverted microscope GAPDH
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