To assess total phenolic contents, total flavonoid contents (TPC, TFC), DPPH scavenging and antidiabetic (antiglycation and alpha amylase hindrance) activities of Moringa oleifera (MO) leaves, bioassay guided fractionation was used with thin layer chromatography profile to isolate saponin fraction (SF). The TPC and TFC were 9.7-12.8 GAE and 5.3-9.3 CE (g/100 g dry weight), respectively and aqueous and methanol fractions were more effective. DPPH scavenging activity ranged 69.5-83.1%. SF displayed noteworthy antioxidant ability (p<0.046) and it was leading inhibitor of glycation (p<0.041) after MeOH with 48.1% restriction. Enzyme inhibition by MO fractions was negligible (3.4-9.2%) and it was less impressive by SF as compared to MeOH extract. Percent reduction in enzyme activity by SF was nonsignificant (p>0.198), nullifying its concentration dependent impact.