Abstract:
-Chilli is a major cash crop of Pakistan and is prone to various bacterial diseases. Therefore, the current study was planned to elucidate the resistant chilli germplasms against local chilli putative bacterial isolates. For isolation of bacteria, chilli disease samples were collected from Taxila, Mianwali, Islamabad, Chakwal, and Rawalpindi during 2021-22. In addition, seed borne bacterial infection percentage and isolation from different chilli varieties was also performed. In total, 9 isolates were obtained out of which 5 were obtained from infected leaf samples while the rest were isolated by seeds. Seed infection varied from 10 to 20 % in different chilli varieties. Biochemical assays were performed for the characterization of pathogens. The two well characterized isolates GP (plate poison technique) and APO (Pseudomonas syringae) were used in chilli gerplasm screening. Leaf detach and seedling inoculations methods were used for pathogenicity assays. Hot pepper and Gaula (sweet pepper) were found resistance against GP and APO respectively in leaf detach assay. Out of three varieties (TAX, Sky Star and Golden Hot), TAX resistance against Xanthomonas vesicatoria while Sky star found resistance against Pseudomonas syringae in seedling inoculation assay. GP and APO antibiotic sensitivity (erythromycin penicillin, streptomycin, and tetracycline) assays were also performed by disc diffusion method. It was found that tetracycline and streptomycin were effective against GP and APO respectively. Our data showed that Xanthomonas vesicatoria and Pseudomonas syringae were quite common in chilli field plantations and chilli germplasm must be screened against these bacteria before sowing to avoid future disease spread.
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DOI:
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Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022