The seed extract of Oroxylum indicum suppresses cell proliferation, migration and promotes apoptosis in cervical cancer cells | [Pakistan Journal of Pharmaceutical Sciences • 2022]

Author(s):

1. Benjaporn Buranrat: Faculty of Medicine, Mahasarakham University,Muang, Maha Sarakham,Thailand

2. Supavadee Boontha: School of Pharmaceutical sciences, University of Phayao,Maeka, Phayao,Thailand

Abstract:

The herb Oroxylum indicum has been used for treating several diseases and also has anticancer activity. This research examined the anticancer effects of ethanolic extract of O. indicum seeds on HeLa cervical cancer cells and investigates underlying mechanisms. The data indicated that the extract inhibited HeLa cells growth with low IC50 values and arrested the cell cycle at G0/G1 phase at a dose of 50 µg/mL. Moreover, this extract produced a marked increase in the apoptosis of cancer cells which was demonstrated by acridine orange/ethidium bromide (AO/EB) staining and was significant at a dose of 250 µg/mL for 24 h. Correlating with apoptotic data by flow cytometric analysis, the results indicated that viable cells were significantly reduced and late apoptotic cells were induced starting at the dose of 50 µg/mL of the extract. These extracts significantly induced cancer cell apoptosis which was mediating through reduction of mitochondrial function by stimulating intracellular reactive oxygen species (ROS) formation. Furthermore, the extract caused inactivation of cervical cancer cell migration and was detected with the wound healing method. The data in this study strongly indicated that O. indicum seed extract has powerful activity against cervical cancer.

Page(s): 553-559

DOI: 10.36721/PJPS.2022.35.2.REG.553-559.1

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 35, Issue: 2, Year: 2022

Keywords:

Apoptosis , Migration , cell cycle arrest , mitochondrial membrane potential , Oroxylum indicum

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