Uncaria Gambir Roxb (Bajakah) Effects on tumor growth, apoptosis, inflammation, and angiogenesis of DMBA-Induced mammary carcinoma in rat: An immunohistochemistry study | [Rawal Medical Journal • 2025]

Author(s):

1. Prabudi Prabudi: Department of Surgical Oncology Hasan Basry Hospital, South Kalimantan

2. Nia Kania: Department of Pathology Anatomy, Faculty of Medicine and Health Sciences, Lambung Mangkurat University, Ulin General Hospital, Banjarmasin, South Kalimantan

3. Eko Suhartono: Department of Medical Chemistry/Biochemistry, Faculty of Medicine and Health Sciences, Lambung Mangkurat University, Banjarbaru, South Kalimantan

4. Didik Dwi Sanyoto: Departement of Biomedical, Anatomy Division, Faculty of Medicine and Health Sciences, Lambung Mangkurat University, Banjarmasin, Indonesia

Abstract:

Objective: To investigate the antitumor effects of U. Gambir extract on 7, 12-dimethylbenz [a] anthracene (DMBA)-induced mammary carcinoma in Wistar rats. Methodology: This experimental study included 30 female rats divided in five groups receiving different U. Gambir extracts at 200 mg/kg, 600 mg/kg, 1000 mg/kg body weight (BW), and positive control (capecitabine 65mg/kg BW). Apoptosis, inflammation, and angiogenesis induction were assessed by immunohistochemistry, and quantifying biomarkers caspase-3, NF-KB, and VEGFR-2 expressions using the Histoscore method. Statistical analyses included one-way ANOVA (tumor volume) with Bonferroni post hoc and chi-square tests for biomarker expression. Results: The 600 mg/kg BW U. Gambir group exhi-bited significant tumor growth inhibition (p0.05). VEGFR-2 expression is no different compared to the control group. Conclusions: These findings suggest that U. Gambir extracts have antitumor activity by decreasing tumor volume. However, further research is essential to def-ine the mechanistic pathways and identify active com-ponents.

Page(s): 762-765

DOI: DOI not available

Published: Journal: Rawal Medical Journal, Volume: 50, Issue: 3, Year: 2025

Keywords:

Breast Cancer , caspase3 , NFkB , U Gambir , tumor growth , VEGFR2

References:

[1] Bray F,Laversanne M,Sung H,Ferlay J,Siegel RL,Soerjomataram I .2024 .GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin, 74 : 229-263.

[2] .2014 .. , : .

[3] Greenlee H,Balneaves LG,Carlson LE,Cohen M,Deng G,Hershman D .2014 .Clinical practice guidelines on the use of integrative therapies as supportive care in patients treated for breast cancer. J Natl Cancer Inst Monogr, : 346-358.

[4] Knecht K,Kinder D,Stockert A .2020 .Biologically-Based Complementary and Alternative Medicine (CAM) Use in Cancer Patients: The Good, the Bad, the Misunderstood. Front Nutr, 6 : 196-9.

[5] Al-Hawaris .2023 .Review: potency of borneo endemic and typical plants as anti-cancer medicines. Jurnal Kesehatan Pasak Bumi Kalimantan, 5 : 37-53.

[6] Nasrul W,Wardianto D,Rizalman D .2020 .Program Kemitraan Masyakat Produksi dan Pemasaran Gambir (Uncaria Gambir Roxb). Jurnal Pengabdian Untuk Mu Nege RI, 4 : 187-191.

[7] Meiliana A,Dewi NM,Wijaya A .2016 .Cancer Stem Cell Hypothesis: Implication for Cancer Prevention and Treatment. Indonesian Biomed J, 8 : 21-5.

[8] Syarifah S,Widyawati T,Rita Anggraini D,Sari Wahyuni A,Indah Sari M. A .2019 .Anticancer activity of uncaria gambir roxb on T47D breast cancer cells. J Physics, 10 : 1742.

[9] Hassan AK,El-Kalaawy AM,Abd El-Twab SM,AlBlihed MA,Ahmed OM .2023 .Hesperetin and Capecitabine Abate 1, 2 Dimethylhydrazine-Induced Colon Carcinogenesis in Wistar Rats via Suppressing Oxidative Stress and Enhancing Antioxidant, Anti-Inflammatory and Apoptotic Actions. Life, 13 : 1-18.

[10] Li J,Malakhova M,Mottamal M,Reddy K,Kurinov I,Carper A .2012 .Norathyriol suppresses skin cancers induced by solar ultraviolet radiation by targeting ERK kinases. Cancer Res, 72 : 260-70.

[11] Al-Tinoz A,El-Maci M,Ozpinar İ,A İ,Cancers Systemic,Huang Y,Xie Z,Jiwa H,Zhang J,Wang Y,Xu J .2023 .Asiatic acid affects the growth of human osteosarcoma cells by regulating PI3K/AKT and NF-κB dual signal pathways. J Funct Foods, 111 : 105885.

Citations

Downloads

Views