Abstract:
The modern allopathic medicines are not only costly but also create many side effects. Due to overuse of modern drugs, the resistance against antimicrobial agents is rising. Dueto ease in use, social acceptance, cost effectiveness, natural products are gaining more popularity worldwide.The current study was designed, to evaluate the biological, phytochemical, and biochemical potential of different parts of Cyamopsis tetragonoloba.The antibacterial, antiviral, antibiofilm and antioxidant activities of edible and inedibleparts of C.tetragonoloba were studied.Disc diffusion method was performed for anti bacterial activity.Antibacterial assay was performed against five bacterial strains; E.coli, K.pneumoniae, S.aureus, P.aeruginosa, P.vulgaris and two MDR strains.Antibiofilm potential was checked in round 96-well plate by using crystal violet dye and taking OD at 630nm. The antiviral screening against AIV H9N2 and IBV viruses wereperformed by Hemagglutination test (HA). The antioxident potential was measured by DPPH and FRAP assays. The phytochemical screening of plant C. tetragonoloba was done by Qualitative tests. The extracts were made in MtOH, EtOH,n-hexane, Ethylacetate and distilled water and evaporated in rotary evaporator.Antibacterial assay was performed against five bacterial strains; E.coli, K.pneumoniae,S.aureus, P.aeruginosa, P.vulgaris and two MDR strains.All the extracts have shownsignificant antibacterial potential against bacterial strains. In case of edible part MtOHextract was the most active while in case of inedible part the ethyl acetate extract have shown high antibacterial potential and their Zone of inhibition (ZoIs) were 11.5mm and 13.5 mm respectively. The antiviral screening against AIV H9N2 and IBV viruses wereperformed. The extracts have shown great antiviral activities. The EtOH and ethyl acetateextracts of edible part were highly active against AIV H9N2 while in case of inedibleparts, Aqueous extract was active. In case of anti-IBV activity, most of the extracts wereactive. The EtOH, n-hexane and Ethyl acetate extracts of edible part were highly active. While the results ofinedible parts showed that, MtOH and Aqueous extracts have shownmax activity. The study of antibiofilm activity revealed that MtOH and Aqueous extractsof edible and inedible part have shown good antibiofilm potential. In antioxidant activity including FRAP and DPPH most of the extracts were active except Aqueous.The ethyl acetate extract of edible an inedible part has shown max activity in FRAP assay. In caseof DPPH, MtOH extract of edible part and EtOH extract of inedible parts have shownmax activity. The phytochemical screening of plant C. tetragonoloba has revealed many chemical compound. The study concluded that the cholistani desert plant C. tetragonoloba was found to be rich source of biological and pharmacological agent and itcan beused both in crude and purified form.
Page(s):
99-99
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023