Aberrant expression of CD markers on flow cytometric analysis in suspected patients of leukemias and lymphomas: a single-centered study | [Journal of Sharif Medical & Dental College (JSMDC) • 2022]

Author(s):

1. Hajrah Syndeed Pal: Sharif Medical & Dental College,Sharif Medical City. Sharif Medical City Road, Off Raiwind Road, Jati Umra, Lahore 54000,Pakistan

2. Natasha Ali: Sharif Medical & Dental College,Sharif Medical City. Sharif Medical City Road, Off Raiwind Road, Jati Umra, Lahore 54000,Pakistan

3. Muhammad Asif Naveed: Department of Pathology University of Health Sciences, Lahore,Pakistan

4. Umera Saleem: Sharif Medical & Dental College,Sharif Medical City. Sharif Medical City Road, Off Raiwind Road, Jati Umra, Lahore 54000,Pakistan

5. Yusra Rashid: Sharif Medical & Dental College,Sharif Medical City. Sharif Medical City Road, Off Raiwind Road, Jati Umra, Lahore 54000,Pakistan

6. Mujtaba Ashal Pal: Sharif Medical & Dental College,Sharif Medical City. Sharif Medical City Road, Off Raiwind Road, Jati Umra, Lahore 54000,Pakistan

7. Shabbir Hussain: Sharif Medical & Dental College,Sharif Medical City. Sharif Medical City Road, Off Raiwind Road, Jati Umra, Lahore 54000,Pakistan

Abstract:

Objective: To determine the frequencies and pattern of aberrant CD markers expression in Pakistani patients of leukemias and lymphomas. Methodology: It was a cross-sectional study conducted from April to September 2020 at the Department of Hematology and Transfusion Medicine, Aga Khan University Hospital, Karachi. Peripheral blood/bone marrow samples from patients of suspected hematological malignancies subjected to flow cytometric evaluation were included. Samples were lysed, tested for viability, and analyzed through 5-colour flow cytometer (FC500). Data was entered in Statistical Package for the Social Sciences (SPSS) version 22. Results: A total of 262 patients were enrolled. Male to female ratio in the study was determined to be 1.5:1. Further categorization showed acute leukemias comprised 75.78% of the total cases whereas chronic lymphoproliferative disorders were 22.26%. The overall presence of aberrant markers was 22.8% among all cases. The percentage of aberrant markers expression was higher in leukemic patients as compared to lymphoma patients. Conclusion: Aberrant expression of CD markers is a common entity. Their possible presence should be considered and evaluated while determining the lineage for acute leukemias specifically according to the latest WHO criteria through flow cytometry.

Page(s): 88-91

DOI: DOI not available

Published: Journal: Journal of Sharif Medical & Dental College (JSMDC), Volume: 8, Issue: 2, Year: 2022

Keywords:

Flow cytometry , Lymphomas , Leukemias

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