An improved, simple, inexpensive and highly flexible hydroponic setup for root mitochondria isolation from Arabidopsis and nicotiana pants. | [Pakistan Journal of Botany • 2015]

Author(s):

1. Muhammad Waqar Hameed: Dr. Panjwani Center for Molecular Medicine and Drug Research, University of Karachi, Karachi, Pakistan; Max Planck Institute of Molecular Plant Physiology, Am Muhlenberg 1, 14476 Potsdam-Golm, Germany

2. Aziz-Ud-Din : Department of Genetics, Garden Campus, Hazara University, Mansehra, Pakistan; Max Planck Institute of Molecular Plant Physiology, Am Muhlenberg, Potsdam-Golm, Germany

Abstract:

Hydroponic setups are frequently developed and improved as they are convenient platforms for studying whole plant physiology. Mostly, the available systems produce small amounts of plant material and are therefore, unsuitable for studies requiring large quantities of plant material like isolation of mitochondria. To address this issue, we have modified a hydroponic setup that can sustain hundreds of Arabidopsis and tobacco plants until adult plants are established. The setup is very flexible and easy to construct. It is based on the use of recyclable and sterilizable plastic-net-pots and media containers, which are easily available from the local suppliers. The modified seed-pots and styrofoam sheets facilitate the transfer and harvesting of seedlings. We have used the Percoll based two-step density gradient centrifugation method for the isolation of root mitochondria from the hydroponically grown plants.

Page(s): 2037-2043

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 47, Issue: 5, Year: 2015

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